Nitrofurans are synthetic broad-spectrum antibiotics,which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties.they had been also used as growth promoters in the pig production, poulty and fish sector. In long term studies with experimental animals, the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics.This has led to a prohibition of naturfurans for the treatment used for food production.The naturafuran drugs furantadone,nitrofurantion and nitrofurazone were banned from use in food animal production in the EU in 1993, and the use furazolidone was prohibited in 1995.
The analysis of residues of nitrofuran drugs needs to be based on the detection of the tissue bound matabolites of the nitrofuran parent drugs.Science the parent drugs are very rapidly metabolized, they are not detectable for a short time after treatment. The tissue bound nitrofuran metabolites are detectable for a long time after administration and therefore they are used for the detection of the abuse of nitrofurans. Nitrofuran metabolites are found after administration of Furanltadone(AMOZ), Furazolidone(AOZ), Nitrofurazone(SEM), Nitrofurantoin (AHD).
AMOZ-residues are determined most commonly by LC-UV,LC-MS techniques. Enzyme immunoassay,compared with chromatographic methods, show considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement.
Using the RIDSCREEN Nitrofuran (AMOZ) test, it is possible to detect AMOZ in fish, shrimps, honey, meat (chicken,pork,beef) and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)
Specifications:96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1--8.1ppb
Detection limit:
honey, meat and liver……………………… 0.2ppb
fish and shrimp …………………………… 0.3ppb
Sample preparation time:
fish and shrimp ……………………………2h
honey,meat and liver ………………………2h
Recovery rate:
fish and shrimp ……………………………75-105%
honey,meat and liver……………………65-95%
Cross-reations:
AMOZ ……………………………………… 100%
AOZ ……………………………………… ﹤0.1%
AHD ……………………………………… ﹤0.1%
SEM ……………………………………… ﹤0.1%
Test implemention time: 80 min
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